Hello to all, I am a student in Master1 of cellular and molecular biology. While looking for how the genetic code was discovered (and especially the notion of degeneracy), I came across a historical article from 1961 (General Nature of Genetic Code for Proteins, Crick et al.). I am trying to understand how the T4 rII system used in the experimental model in the article works. I found a good video in English explaining this but I am not sure I understand the difference between K and B cultures very well. Could someone please enlighten me? Here is the link to the video: Have a nice day, Jérome.
We have to watch a hour long video just to get to your question? Couldn't you just flesh out your question a bit more here?
Hello Origin, The portion of the video I am talking about is spreading from 1'40'' to 6'10'', so less than 5 minutes (the part about the T4 rII system that I don't really understand). I wanted to know what are the differences between the culture medium K and B... I don't understand how they differ at a cellular level, ones being "host" for recombination and other used just to know whether the rII gene is (non)-functional. Do you have any idea?
Wow, you’re giving me flashbacks to my university genetics lectures! Please Register or Log in to view the hidden image! It’s been almost 30 years since I studied bacteriophage transduction of E.coli and how you can use it to determine genetic structure and gene order. It’s a very specific teaching method and a very specific question that I don’t have the time to re-learn in order to answer you. Sorry. A study session with your fellow classmates would be the best approach.