Methods Of Biological Immortality 2019

[Vmedvil]

I have found a way to make yourself biological immortal but it will be expensive at 12 cents a base pair, the human genome is 3.2 billion base pairs theoretically you could construct your entire genome in plasmids there would be 1,066,667 plasmids if each plasmid contained one gene which the average length of a gene is 3000 bp. If you constructed your entire genome within Retroviral Vectors it would cost 448 million dollars per person, then you would have a active vector containing each one of your genes that are undamaged then you could use this synthetic DNA template to reintegrate DNA as it mutated keeping you alive forever. This is with current day technology that biological immortality could be achieved then like the storing your DNA method, you could live forever without end as long as the Viral Vectors were kept away from things like radiation always having a perfect set of your own synthetic DNA awaiting to be transferred into your body at a moment's notice as any gene is damaged via mutation. This would reverse and permanently stop the aging process your cells always being in perfect health via genetic immortality being able to produce every protein without natural mutation being a genetic code that doesn't change over time. You could live to be a million-million years old and never age a bit as the genetic code could always code for the proper proteins like a 18 year old. I dub this new method "Genetic Reconstruction, Immortality" and put a price tag of 600 million dollars on the gene therapy per treatment which will make you never die via aging.

 

[Hercules Rockefeller]

Okay, lots of problems here.

I have found a way to make yourself biological immortal but it will be expensive at 12 cents a base pair, the human genome is 3.2 billion base pairs theoretically you could construct your entire genome in plasmids there would be 1,066,667 plasmids if each plasmid contained one gene which the average length of a gene is 3000 bp.

Huh? Only around 1% of the genome is genes, ie. DNA that encodes proteins. So, say about 32 million base pairs. The rest (99%) is non-coding. These non-coding DNA sequences are often highly repetitive and can be exceedingly difficult to synthesize and sub-clone into vectors. Currently it’s only possible to synthetically create the simplest of bacterial and viral genomes (which are much smaller than mammalian genomes, and contain virtually no highly repetitive DNA such as that found in the genomes of plants and animals). The human genome is a loooooooooooong way off from being synthetically generated. That’s Star Trek-style fantasy at this stage.

But that’s not to say non-coding DNA is unimportant. Nobody (who knows what they’re talking about) refers to “junk DNA” any more. Far from it. Non-coding DNA contains sequences that contribute to the formation of chromosomal structures, like centromeres and telomeres. Non-coding DNA contains transcriptional regulatory sequences that influence the activity of promoters.

If you constructed your entire genome within Retroviral Vectors it would cost 448 million dollars per person, then you would have a active vector containing each one of your genes that are undamaged then you could use this synthetic DNA template to reintegrate DNA as it mutated keeping you alive forever.

How do you propose to integrate new DNA into every cell in your body? The only way to do this is to clone an organism. ie. regenerate it from scratch from a single cell that you’ve manipulated to your liking. But then, that’s an entirely new organism, not a recreation of the original. (Organism = genetics + environment)

This would reverse and permanently stop the aging process your cells always being in perfect health via genetic immortality being able to produce every protein without natural mutation being a genetic code that doesn't change over time.

Mutation is not the sole mechanism behind ageing. There are other mechanisms, such as telomere shortening and DNA methylation.

 

[Vmedvil]

Okay, lots of problems here.



Huh? Only around 1% of the genome is genes, ie. DNA that encodes proteins. So, say about 32 million base pairs. The rest (99%) is non-coding. These non-coding DNA sequences are often highly repetitive and can be exceedingly difficult to synthesize and sub-clone into vectors. Currently it’s only possible to synthetically create the simplest of bacterial and viral genomes (which are much smaller than mammalian genomes, and contain virtually no highly repetitive DNA such as that found in the genomes of plants and animals). The human genome is a loooooooooooong way off from being synthetically generated. That’s Star Trek-style fantasy at this stage.

But that’s not to say non-coding DNA is unimportant. Nobody (who knows what they’re talking about) refers to “junk DNA” any more. Far from it. Non-coding DNA contains sequences that contribute to the formation of chromosomal structures, like centromeres and telomeres. Non-coding DNA contains transcriptional regulatory sequences that influence the activity of promoters.



How do you propose to integrate new DNA into every cell in your body? The only way to do this is to clone an organism. ie. regenerate it from scratch from a single cell that you’ve manipulated to your liking. But then, that’s an entirely new organism, not a recreation of the original. (Organism = genetics + environment)



Mutation is not the sole mechanism behind ageing. There are other mechanisms, such as telomere shortening and DNA methylation.

1) I was talking about just synthesizing the entire DNA structure including the non-coding elements and putting them into plasmids.

2)I never said "Junk DNA" or anything about it.

3) Retroviral Vectors with the VSV-G gene go into every cell and integrate the genetic elements between the LTR5' and LTR3' into the genome that are as long as 9000 bp.

4) Just drop in the telomeres and such from a earlier aged template too, which the telomeres are just DNA too.

 

[Hercules Rockefeller]

1) I was talking about just synthesizing the entire DNA structure including the non-coding elements and putting them into plasmids.

As I said, this cannot be done with current technologies. At this time, artificially synthesizing genomes is restricted to the simplest of bacterial and viral genomes.

2)I never said "Junk DNA" or anything about it.

You didn’t, but I brought it up because you don’t seem to appreciate or understand the difference between exons and non-coding DNA, and their relative abundance in the genome.

3) Retroviral Vectors with the VSV-G gene go into every cell and integrate the genetic elements between the LTR5' and LTR3' into the genome that are as long as 9000 bp.

Are you trying to say that if a retroviral vector is parenterally injected into your body it will modify every one of your cells? If you are, then that’s total nonsense.

4) Just drop in the telomeres and such from a earlier aged template too, which the telomeres are just DNA too.

Yep easy. Just drop them all in (if you’re Dr. Beverly Crusher from Star Trek: The Next Generation).

 

[Vmedvil]

You didn’t, but I brought it up because you don’t seem to appreciate or understand the difference between exons and non-coding DNA, and their relative abundance in the genome.



Are you trying to say that if a retroviral vector is parenterally injected into your body it will modify every one of your cells? If you are, then that’s total nonsense.

1) Even the non coding regions are just DNA, I know you said they have repeats then them which is a issue with DNA synthesis, I can definitely understand that as that's why a DNA synthesis company I have tried to get to make the Chickenpox Capsid won't do it because of the repeats in that gene which is a issue with synthetic gene synthesis, someone will eventually figure it out to maximize profits and such.

2) I am not saying that a single virion will transfect every cell in the body, what I am saying is that if you have virions equal to the number of cells in the body they will transfect all the cells in the body as along as the virions express the VSV-G glycoprotein.

 

[Beaconator]

Worth a try but I certainly won’t be your test dummy. Won’t pay for it either.

but it seems like exacting DNA and reinserting it would lead to the exact same time of death.

 

[billvon]

I have found a way to make yourself biological immortal but it will be expensive at 12 cents a base pair, the human genome is 3.2 billion base pairs theoretically you could construct your entire genome in plasmids there would be 1,066,667 plasmids if each plasmid contained one gene which the average length of a gene is 3000 bp. If you constructed your entire genome within Retroviral Vectors it would cost 448 million dollars per person, then you would have a active vector containing each one of your genes that are undamaged then you could use this synthetic DNA template to reintegrate DNA as it mutated keeping you alive forever.

That's not what most people die of, so no, that wouldn't make you live forever.

 

[Vmedvil]

Worth a try but I certainly won’t be your test dummy. Won’t pay for it either.

but it seems like exacting DNA and reinserting it would lead to the exact same time of death.

That's not what most people die of, so no, that wouldn't make you live forever.

Why does your skin age and lose its elasticity... because it is not producing a certain protein that at a younger age it had produced in large quantities by restoring the genetic age to a younger age all the organs and tissues will always produce the proteins they had produced when you are younger that kept the tissues and organs healthy, that is the premise behind this.

 

[origin]

Why does your skin age and lose its elasticity... because it is not producing a certain protein

Nope. You can't simply add one 'certain' protein to your skin and have your skin no longer age. This sounds like a crappy Olay Skin Cream commercial claim.

 

[billvon]

Why does your skin age and lose its elasticity... because it is not producing a certain protein that at a younger age it had produced in large quantities by restoring the genetic age to a younger age all the organs and tissues will always produce the proteins they had produced when you are younger that kept the tissues and organs healthy, that is the premise behind this.

Nope. Again, there are a lot of reasons for aging. One is that proteins that are hard to break down (think of them as "ashes") accumulate within cells and eventually make them almost inert. Changing the genome of the cell will do nothing to help that. Another problem with your skin is that although you shed epidermis, your dermis contains both dead and living cells. Over time the dead cells accumulate and make your skin less elastic. You can't "restore the genetic age" of a dead cell. It's dead.

 

[Beaconator]

Why does your skin age and lose its elasticity... because it is not producing a certain protein that at a younger age it had produced in large quantities by restoring the genetic age to a younger age all the organs and tissues will always produce the proteins they had produced when you are younger that kept the tissues and organs healthy, that is the premise behind this.

If genetic material were stored at birth then given at a certain age you might have a chance at making life longer but you don’t seem to have this aspect in mind.

 

[DaveC426913]

This is with current day technology...

None of this is current day technology.
None of this is even science fiction.
It's hand-waving.

For example. There's just a wee bit of magic between "we have this protein" and "we insert it into all trillion skin cells and it miraculously expresses itself".

And I'm a little suss about the coherency of the thought process too.

You've already got a dollar value? Where do you get "12 cents per base pair" from, and do you just scale it up times the number of base pairs - as if it's manual labour or something?

These are rhetorical questions.

 

[billvon]

If genetic material were stored at birth then given at a certain age you might have a chance at making life longer but you don’t seem to have this aspect in mind.

Uh - OK. My kids have genetic material stored since birth, but it's not to "extend their life" or any such nonsense.

 

[Beaconator]

Uh - OK. My kids have genetic material stored since birth, but it's not to "extend their life" or any such nonsense.

What kind of nonsense is it for then?

 

[billvon]

What kind of nonsense is it for then?

Cord blood is used to treat a few dozen diseases, often diseases that damage or destroy the immune system or the bone marrow. They are also useful in treating various anemias, metabolic disorders and cancers. It is likely that other treatments will be developed using cord blood in the future now that it has become more available.

 

[Beaconator]

Cord blood is used to treat a few dozen diseases, often diseases that damage or destroy the immune system or the bone marrow. They are also useful in treating various anemias, metabolic disorders and cancers. It is likely that other treatments will be developed using cord blood in the future now that it has become more available.

Oh yeah I forgot about all that. Can’t remember where I heard it first. Was just joking when I said nonsense.